CAS NO: 57-13-6

MOLECULAR FORMULA: CH4N2O

MOLECULAR WEIGHT : 60.06

 

Description
Synonyms Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine
Product Information
CAS number 57-13-6
Grade ACS,Reag. Ph Eur
Hill Formula CH₄N₂O
Chemical formula CO(NH₂)₂
Molar Mass 60.05 g/mol
HS Code 3105 10 00
Structure formula Image
Quality Level MQ200
Physicochemical Information
Density 1.32 g/cm3 (20 °C)
Melting Point 134 °C
pH value 7.5 – 9.5 (480 g/l, H₂O, 25 °C)
Vapor pressure <0.1 hPa (25 °C)
Bulk density 720 – 760 kg/m3
Solubility 1000 g/l
Toxicological Information
LD 50 oral LD50 Rat 8471 mg/kg
LD 50 dermal LD50 Rat 8200 mg/kg
Safety Information according to GHS
RTECS YR6250000
Storage class 10 – 13 Other liquids and solids
WGK WGK 1 slightly hazardous to water
Disposal 3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Storage and Shipping Information
Storage Store at +15°C to +25°C.
Specifications
Assay (ex N) 99.0 – 100.5 %
Assay (ex N, calc. on dried substance) 99.0 – 101.5 %
Purity (DSC (differential scanning calorimetry)) ≥ 99.5 mol%
Identity (IR-spectrum) passes test
Appearance of solution (10 %; Wasser; color) colourless
Appearance of solution (10 %; Wasser; clarity) clear
In water insoluble matter ≤ 0.003 %
Acidity, Alkalinity ≤ 0.0005 meq/g
Melting point (DSC) 132 – 135 °C
NH₄ (Ammonium) ≤ 0.0500 %
Chloride (Cl) ≤ 0.0005 %
Sulfate (SO₄) ≤ 0.001 %
Heavy metals (as Pb) ≤ 0.0004 %
Biuret ≤ 0.05 %
Sulfated ash (600 °C) ≤ 0.005 %
Cu (Copper) ≤ 0.0001 %
Fe (Iron) ≤ 0.0001 %
Pb (Lead) ≤ 0.0002 %
Loss on Drying (105 °C) ≤ 1.0 %
Corresponds to ACS, Reag. Ph Eur

Description

Urea can be used as a detergent, similar to guanidine hydrochloride (sc-202637), to denature proteins at high concentrations, or in cell culture to increase the osmolarity. Transformed microorganisms often produce inclusion bodies, which are insoluble, inactive bodies, that form during recombinant protein production. These inclusion bodies can be denatured with urea or guanidine hydrochloride. The compound prefers to interacts with non-polar and aromatic sides and can be used in polyacrylamide gels containing urea to fractionate low molecular weight DNA or RNA.

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