Urea

Name: Urea
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Categories: Food & Nutraceutical, Personal Care and Cosmatic, Pharmaceuticals

CAS NO: 57-13-6

MOLECULAR FORMULA: CH₄N₂O

MOLECULAR WEIGHT : 60.06

Description
Synonyms	Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine
Product Information
CAS number	57-13-6
Grade	ACS,Reag. Ph Eur
Hill Formula	CH₄N₂O
Chemical formula	CO(NH₂)₂
Molar Mass	60.05 g/mol
HS Code	3105 10 00
Structure formula Image
Quality Level	MQ200
Physicochemical Information
Density	1.32 g/cm3 (20 °C)
Melting Point	134 °C
pH value	7.5 – 9.5 (480 g/l, H₂O, 25 °C)
Vapor pressure	<0.1 hPa (25 °C)
Bulk density	720 – 760 kg/m3
Solubility	1000 g/l
Toxicological Information
LD 50 oral	LD50 Rat 8471 mg/kg
LD 50 dermal	LD50 Rat 8200 mg/kg
Safety Information according to GHS
RTECS	YR6250000
Storage class	10 – 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3
Disposal	Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Storage and Shipping Information
Storage	Store at +15°C to +25°C.
Specifications
Assay (ex N)	99.0 – 100.5 %
Assay (ex N, calc. on dried substance)	99.0 – 101.5 %
Purity (DSC (differential scanning calorimetry))	≥ 99.5 mol%
Identity (IR-spectrum)	passes test
Appearance of solution (10 %; Wasser; color)	colourless
Appearance of solution (10 %; Wasser; clarity)	clear
In water insoluble matter	≤ 0.003 %
Acidity, Alkalinity	≤ 0.0005 meq/g
Melting point (DSC)	132 – 135 °C
NH₄ (Ammonium)	≤ 0.0500 %
Chloride (Cl)	≤ 0.0005 %
Sulfate (SO₄)	≤ 0.001 %
Heavy metals (as Pb)	≤ 0.0004 %
Biuret	≤ 0.05 %
Sulfated ash (600 °C)	≤ 0.005 %
Cu (Copper)	≤ 0.0001 %
Fe (Iron)	≤ 0.0001 %
Pb (Lead)	≤ 0.0002 %
Loss on Drying (105 °C)	≤ 1.0 %
Corresponds to ACS, Reag. Ph Eur

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Description

Urea can be used as a detergent, similar to guanidine hydrochloride (sc-202637), to denature proteins at high concentrations, or in cell culture to increase the osmolarity. Transformed microorganisms often produce inclusion bodies, which are insoluble, inactive bodies, that form during recombinant protein production. These inclusion bodies can be denatured with urea or guanidine hydrochloride. The compound prefers to interacts with non-polar and aromatic sides and can be used in polyacrylamide gels containing urea to fractionate low molecular weight DNA or RNA.