Disodium Edetate

Name: Disodium Edetate
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Categories: Food & Nutraceutical, Oral Care, Personal Care and Cosmatic, Pharmaceuticals

CAS NO: 6381-92-6

MOLECULAR FORMULA :- C₁₀H₁₄N₂Na₂O₈2H₂O

MOLECULAR WEIGHT :- 372.24 g/ mol

SPECIFICATION	IP	BP	USP
Description	A white ,crystalline powder, Odourless	White or almost white, crystalline powder.	White or almost white, crystalline powder.
Solubility	–	Soluble in water, practically insoluble in ethanol (96 %)	–
Identification A. IR B. Reaction C. Reaction D. Reaction	A. Must comply with the disodium edetate RS B. No precipitate is produced. C. No precipitate is produced. D. It gives reaction of sodium salt.	A. Must comply with the disodium edetate RS B. No precipitate is produced. C. No precipitate is produced. D. It gives reaction of sodium salt.	A. Must comply with the disodium edetate RS B. The red color is discharge, leaving a yellowish solution C. It gives reaction of sodium salt.
Appearance of solution	5 % w/v solution in carbon dioxide free water is Clear & colorless	Clear & colorless	–
pH	4.0-5.5 ( 5 % w/v solution )	4.0-5.5 ( 5 % w/v solution )	4.0-6.0
Loss on Drying	–	–	8.7 %-11.4 %
Calcium	–	–	No precipitate is formed.
Impurity A Limit of nitrilotriacetic acid(USP)	NMT 0.1 %	NMT 0.1 %	NMT 0.1 %
Heavy metals	NMT 20 ppm	–	NMT 50 ppm
Iron	NMT 80 ppm	NMT 80 ppm	–
Assay	98.5 %-101.0 %	98.5 %-101.0 %	99.0 %-101.0 %(on Dried basis)

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Description

EDTA, disodium salt, dihydrate, for molecular biology is extensively used as a chelator of divalent cations such as Ca²⁺. EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. In molecular biology applications, addition of EDTA minimizes the impact of metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. It also inhibits enzymes, such as metalloproteases, which require divalent cations for activity. EDTA, disodium salt, dihydrate, for molecular biology is an inhibitor of Calpain.

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